Journal: BioMed Research International
Article Title: CUMS Promotes the Development of Premature Ovarian Insufficiency Mediated by Nerve Growth Factor and Its Receptor in Rats
doi: 10.1155/2020/1946853
Figure Lengend Snippet: The expression of TrkA, p75, and FSHR proteins was upregulated by exogenous NGF in ovarian tissue of CUMS rats in vitro . (a) Variations of NGF protein expression in the ovarian tissue of CUMS rats after intervention with exogenous NGF in vitro . (b) Variations of TrkA protein expression in the ovarian tissue of CUMS rats after intervention with exogenous NGF in vitro . (c) Variations of p75 protein expression in the ovarian tissue of CUMS rats after intervention with exogenous NGF in vitro . (d) Variations of FSHR protein expression in the ovarian tissue of CUMS rats after intervention with exogenous NGF in vitro . (e) Protein bands for the control, NGF, FSH, and NGF/FSH groups are shown. The molecular weight of TrkA is 150 kDa, FSHR is 77 kDa, p75 is 75 kDa, NGF is 30 kDa, and GAPDH is 38 kDa. Values are mean ± SD for six samples. ∗ P < 0.05, ∗∗ P < 0.01, compared with the control group; # P < 0.05, ## P < 0.01 compared with the NGF group; & P < 0.05, && P < 0.01, compared with the FSH group.
Article Snippet: A blocking solution was added for incubation at 37°C for 1 h. The following primary antibodies were subsequently used: rabbit polyclonal anti-NGF (1 : 100), mouse monoclonal anti-FSHR (1 : 100; Abcam), rabbit polyclonal anti-TrkA (1 : 100), and mouse monoclonal anti-p75 NGF (1 : 100; Abcam).
Techniques: Expressing, In Vitro, Molecular Weight